Kevin Mouillesseaux, Ph.D.

Postdoctoral Fellow

Contact Info: kevinmou [at] email.unc.edu

Education:

University of California, Los Angeles – PhD, 2011
University of California, San Diego – BS, 2001

Research:

My work focuses on how individual EC within the developing
vasculature resolve multiple simultaneously acting angiogenic stimuli.
At the single-cell level, thresholds of responsiveness to angiogenic
signaling generate heterogeneity along the length of a vessel which is
essential to the proper patterning and function of the vasculature.
Notch and BMP signaling are increasingly gaining attention as
important, context-dependent regulators of angiogenesis. I am trying
to build on recent work in the Bautch lab that revealed BMPs to be
potently pro-angiogenic in the venous vasculature of developing
zebrafish embryos. More recently, we discovered that Notch signaling
may strongly influence the magnitude of a cell’s response to
pro-angiogenic BMP signaling, thereby regulating which cells in a
growing vessel will assume BMP-dependent morphologies. Finally, I am
currently using molecular, cellular, and genetic techniques to
elucidate the molecular mechanism underlying Notch-dependent regulation
of BMP-responsiveness at the single-cell level in vitro and in vivo.

Publications:

Wiley DM*, Mouillesseaux KP*, Meadows SM, Saunders LM, Chong DC,
Barber AT, Cleaver O, Jin S-W, Bautch VL (2014) Notch Modulates
Endothelial Cell Responses to Pro-Angiogenic BMP Signaling and
Selectively Affects Lateral Branching during Angiogenesis. In
submission at Circulation Research *authors contributed equally

Monte E*, Mouillesseaux K*, Chen H, Kimball T, Shuxun R, Wang Y, Chen
J-N, Vondriska T, Franklin S (2013) Systems Proteomics of Cardiac
Chromatin Identifies Nucleolin as a Regulator of Growth and Cellular
Plasticity in Cardiomyocytes. AJP-Heart and Circ Phys, 305(11) 1624-38.
*authors contributed equally

Chappell JC, Mouillesseaux KP, Bautch VL (2013) Flt-1 (Vascular
Endothelial Growth Factor Receptor-1) Is Essential for the Vascular
Endothelial Growth Factor-Notch Feedback Loop During Angiogenesis.
ATVB. PMID 23744993

Mouillesseaux KP, Chen JN (2011) Mutation in utp15 disrupts vascular
patterning in a p53-dependent manner in zebrafish embryos. PLoS One 6
(9): e25013 PMID 21949834

Choi J*, Mouillesseaux KP*, Wang Z, Fiji H, Kinderman S, Otto G,
Giesler R, Kwon O, Chen JN (2011) Aplexone Targets the HMG-CoA
Reductase Pathway and Differentially Regulates Arteriovenous
Angiogenesis. Development 138 (6): 1173-1181, *authors contributed
equally PMID 21307094

Gugiu BG, Mouillesseaux K, Duong V, Herzog T, Hekimian A, Koroniak
L, Vondriska TM, Watson AD (2008) Protein targets of oxidized
phospholipids in endothelial cells. J Lipid Res. 49(3):510-20. PMID
18071189

Gharavi NM, Alva JA, Mouillesseaux KP, Lai C, Yeh M, Yeung W,
Johnson J, Szeto WL, Hong L, Fishbein M, Wei L, Pfeffer LM, Berliner JA
(2007) Role of the Jak/STAT pathway in the regulation of interleukin-8
transcription by oxidized phospholipids in vitro and in atherosclerosis
in vivo. J Biol Chem. 282(43):31460-8 PMID 17726017

Zimman A, Mouillesseaux KP, Le T, Gharavi NM, Ryvkin A, Graeber TG,
Chen TT, Watson AD, Berliner JA (2007) Vascular endothelial growth
factor receptor 2 plays a role in the activation of aortic endothelial
cells by oxidized phospholipids. Arterioscler Thromb Vasc Biol.
27(2):332-8. PMID 17110601

Gharavi NM, Baker NA, Mouillesseaux KP, Yeung W, Honda HM, Hsieh X,
Yeh M, Smart EJ, Berliner JA (2006) Role of endothelial nitric oxide
synthase in the regulation of SREBP activation by oxidized
phospholipids. Circ Res. 98(6):768-76. PMID 16497987

Li R, Mouillesseaux KP, Montoya D, Cruz D, Gharavi N, Dun M,
Koroniak L, Berliner JA (2006) Identification of prostaglandin E2
receptor subtype 2 as a receptor activated by OxPAPC. Circ Res.
98(5):642-50. PMID 16456101

Yeh M, Cole AL, Choi J, Liu Y, Tulchinsky D, Qiao JH, Fishbein MC,
Dooley AN, Hovnanian T, Mouillesseaux K, Vora DK, Yang WP,
Gargalovic P, Kirchgessner T, Shyy JY, Berliner JA (2004) Role for
sterol regulatory element-binding protein in activation of endothelial
cells by phospholipid oxidation products. Circ Res. 95(8):780-8. PMID
15388640

Yeh M, Gharavi NM, Choi J, Hsieh X, Reed E, Mouilleseaux [sic] KP, Cole
AL, Reddy ST, Berliner JA (2004) Oxidized phospholipids increase
interleukin 8 (IL-8) synthesis by activation of the c-src/signal
transducers and activators of transcription (STAT)3 pathway. J Biol
Chem. 279(29):30175-81. PMID 15143062

Mouillesseaux KP, Klimpel KR, Dhar AK (2003) Improvement in the
specificity and sensitivity of detection for the Taura syndrome virus
and yellow head virus of penaeid shrimp by increasing the amplicon size
in SYBR Green real-time RT-PCR. J Virol Methods 111(2):121-7. PMID
12880927