John Chappell, Ph.D.
Contact info: JChappell@unc.edu
University of Virginia, Charlottesville, VA – Ph.D. Biomedical Engineering, 2007
University of Virginia, Charlottesville, VA – M.S. Biomedical Engineering, 2005
University of Virginia, Charlottesville, VA – B.S. Electrical Engineering, 2001
My research focuses on understanding how Flt-1 (VEGFR-1) regulates VEGF signaling during different phases of blood vessel branching morphogenesis. I use a combination of in vitro and in vivo models to manipulate Flt-1 expression and activity to understand how Flt-1 contributes to endothelial heterogeneity that is essential for these unique stages of blood vessel development.
Chappell, J.C., K.P. Mouillesseaux, V.L. Bautch. (2013) Flt-1 (VEGFR-1) is Essential for the VEGF-Notch Feedback Loop during Angiogenesis. Arterioscler Thromb Vasc Biol. Aug;33(8):1952-9.
Chappell J.C., D.M. Wiley, V.L. Bautch. (2012) How blood vessel networks are made and measured. Cells Tissues Organs. 195(1-2):94-107
Chappell J.C., D.M. Wiley, V.L. Bautch. (2011) Regulation of blood vessel sprouting. Semin Cell Dev Biol. Dec;22(9):1005-11.
Randhawa P.K., Rylova S., Heinz J.Y., Kiser S., Fried J.H., Dunworth W.P., Anderson A.L., Barber A.T., Chappell J.C., Roberts D.M., Bautch V.L. (2011) The Ras activator RasGRP3 mediates diabetes-induced embryonic defects and affects endothelial cell migration. Circ Res. 108(10):1199-1208.
Hashambhoy Y.L., J.C. Chappell, S.M. Peirce, V.L. Bautch, F. Mac Gabhann. (2011) Computational modeling of interacting VEGF and soluble VEGF receptor concentration gradients. Front Physiol. 2:62.
Chappell, J.C. and V.L. Bautch. (2010) Vascular Development – Genetic Mechanisms and Links to Vascular Disease. Curr Top Dev Biol. 90:43-72. Invited Review.
Chappell, J.C., S.M. Taylor, N. Ferrara, V.L. Bautch. (2009) Local guidance of emerging vessel sprouts requires soluble Flt-1. Dev Cell. 17(3):377-86.
Chappell, J.C., Song J., Burke C.W., Klibanov A.L., Price R.J. (2008) Targeted delivery of nanoparticles bearing fibroblast growth factor-2 by ultrasonic microbubble destruction for therapeutic arteriogenesis. Small. Oct;4(10):1769-77.
Kappas N.C., G. Zeng, J.C. Chappell, J.B. Kearney, S. Hazarika, C. Patterson, B. Annex, V.L. Bautch. (2008) The VEGF receptor flt-1 spatially modulates flk-1 signaling and blood vessel branching. J Cell Biol. 181(5):847-58.
Chappell, J.C., J. Song, A.L. Klibanov, R.J. Price. (2008) Ultrasonic Microbubble Destruction Stimulates Therapeutic Arteriogenesis via CD18-Dependent Recruitment of Bone-Marrow Derived Cells. Arterioscler Thromb Vasc Biol. 28(6):1117-22.
Chappell, J.C., and R.J. Price. (2006) Targeted therapeutic applications of acoustically active microspheres in the microcirculation. Microcirculation. 13(1):57-70. Review.
Chappell, J.C., A.L. Klibanov, R.J. Price. (2005) Ultrasound-microbubble-induced neovascularization in mouse skeletal muscle. Ultrasound Med Biol. 31(10):1411-22.
Song, J., J.C. Chappell, M. Qi, E.J. Van Gieson, S. Kaul, R.J. Price. (2002) Influence of injection site, microvascular pressure and ultrasound variables on microbubble-mediated delivery of microspheres to muscle. J Am Coll Cardiol. 39(4):726-31.